Quantitative PCR and HER2 Testing in Breast Cancer

Quantitative PCR and HER2 testing in breast cancer: a technical and cost-effectiveness analysis.

We performed a technical and cost-effectiveness analysis of quantitative reverse transcription-polymerase chain reaction (Q-RT-PCR) for the assessment of HER2 in breast cancer. We evaluated 44 frozen and 55 formalin-fixed paraffin-embedded (FFPE) breast carcinoma specimens by Q-RT-PCR, immunohistochemical analysis, and fluorescent in situ hybridization (FISH). Immunohistochemical and FISH analy...

HER2 Testing in Invasive Breast Cancer: a Comparison between Immunohistochemistry and FISH Assays

Analysis of HER2 gene amplification using Differential PCR in breast cancer patients of Isfahan Province

Background: Amplification of HER2 is seen in 20-30% of breast cancer cases. Measurement of HER2 gene amplification appears to be of vital importance in planning the treatment schedule for patients with breast carcinoma. The aim of our study was to evaluate HER2 amplification status in malignant and benign breast tumors by differential PCR (dPCR). Materials and Methods: The genomic DNA was ex...

Optimization of Real Time PCR for Precise Measurement of HER2 Overexpression in Breast Cancer Specimens

Background: Breast cancer is one of the most prevalent malignancies among women in various countries. HER2 overexpression, which is due to different reasons, occurs in 20-30% of breast cancers. HER2 gene encodes an 185kDa transmembrane glycoprotein with 1255 amino acids. This active product triggers downstream intracellular signaling pathways inducing cell proliferation and cell survival. These...

HER2 Testing in Breast Cancer: Opportunities and Challenges

Zusammenfassung Der humane epidermale Wachstumsfaktor-Rezeptor-2 (HER2) wird in 15–25% der Mammakarzinome überexprimiert, üblicherweise auf dem Boden einer Genamplifikation. Ein positiver HER2-Status gilt als ungünstiger Prognosefaktor. Die Aufdeckung der Rolle von HER2 im Tumorwachstum des Mammakarzinoms hat zu der Entwicklung einer anti-HER2-gerichteten Therapie geführt. Der humanisierte mono...